An excerpt from Guns on the Early Frontiers, on the subject of Spanish matchlock muskets in the colonial Americas:
In 1576, a Captain Solis, commander of the Garrison at San Felipe, in the vicinity of what had been the Port Royal of the French Huguenots, executed two Indians, one a chief. Also, Solis had demanded that the neighboring Cusabo bring to the garrison a quantity of of corn and other provisions. Because the food was not promptly forthcoming, the Captain dispatched Moyano and twenty-two soldiers to the Indian villages to seize the needed corn. As the party approached one of the towns, some natives came out to meet it. They explained that their women and children were terrified by the burning slow match carried with every gun, and they indicated that they would cooperate if the soldiers would extinguish their matches. Moyano complied with the request, whereupon the Indians immediately killed all but one of the Spaniards and took the captured matchlocks to the Cusabo town.
Source: Swanton, John R. Early History of the Creek Indians and Their Neighbors. Washington. Bureau of American Ethnology, Bulletin 73, pp 58-59.
I should also point out that the Spanish were the most notorious colonists in the Americas when it came to subjugation and mistreatment of native tribes, and also the most diligent in working to prevent those tribes from acquiring firearms (weird how those go together). Of course, as one might expect, the tribes were able to acquire arms regardless…